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- RIP1 Antibodies
Proteintech
View all (44) RIP1 antibodies
Cite Phospho-RIPK1 (Ser161) Monoclonal Antibody (1B2G1)
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Phospho-RIPK1 (Ser161) Antibody (66854-1-IG) in ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Phospho-RIPK1 (Ser161) antibody (66854-1-Ig, Clone: 1B2G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
66854-1-IG
Applications
Tested Dilution
Publications
Western Blot(WB)
1:5,000
View 7 publications7 publications
Immunohistochemistry (IHC)
-
View 1 publication1 publication
Immunohistochemistry (Paraffin) (IHC (P))
1:500
-
Immunocytochemistry(ICC/IF)
1:400
-
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1B2G1
Immunogen
Peptide
Conjugate
Unconjugated
- CoraLite 594
- CoraLite Plus 488
Form
Liquid
Concentration
1468 µg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Product Specific Information
Aliquoting is unnecessary for -20°C storage.
Target Information
Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:Cell death protein RIP; FLJ39204; OTTHUMP00000015955; receptor (TNFRSF)-interacting serine-threonine kinase 1; Receptor-interacting protein 1; receptor-interacting protein kinase 1; Receptor-interacting serine/threonine-protein kinase 1; RIP-1; RIP1 (CT); serine/threonine-protein kinase RIP
UniProt ID:(Human) Q13546
Entrez Gene ID:(Human) 8737
Molecular Function: 
non-receptor serine/threonine protein kinase
Function(s)
positive regulation of protein phosphorylationapoptotic processactivation of cysteine-type endopeptidase activity involved in apoptotic processI-kappaB kinase/NF-kappaB signalingactivation of JUN kinase activityregulation of tumor necrosis factor-mediated signaling pathwayregulation of necrotic cell deathpositive regulation of necrotic cell deathpositive regulation of interleukin-8 productionpositive regulation of tumor necrosis factor productiontumor necrosis factor-mediated signaling pathwayresponse to tumor necrosis factorTRIF-dependent toll-like receptor signaling pathwaypeptidyl-serine autophosphorylationpositive regulation of apoptotic processpositive regulation of programmed cell deathpositive regulation of I-kappaB kinase/NF-kappaB signalingnegative regulation of I-kappaB kinase/NF-kappaB signalingcellular protein catabolic processpositive regulation of macrophage differentiationpositive regulation of transcription from RNA polymerase II promoterpositive regulation of JNK cascadeprotein autophosphorylationpositive regulation of NF-kappaB transcription factor activityprotein hom*ooligomerizationprotein heterooligomerizationpositive regulation of necroptotic processT cell apoptotic processnecroptotic processregulation of ATP:ADP antiporter activitycellular response to tumor necrosis factorcellular response to growth factor stimulusdeath-inducing signaling complex assemblyapoptotic signaling pathwayextrinsic apoptotic signaling pathwayactivation of cysteine-type endopeptidase activity involved in apoptotic signaling pathwayripoptosome assemblynecroptotic signaling pathwayripoptosome assembly involved in necroptotic processregulation of extrinsic apoptotic signaling pathway via death domain receptorsnegative regulation of extrinsic apoptotic signaling pathway via death domain receptorspositive regulation of hydrogen peroxide-induced cell deathamyloid fibril formationpositive regulation of reactive oxygen species metabolic processnegative regulation of extrinsic apoptotic signaling pathwaypositive regulation of extrinsic apoptotic signaling pathwaynegative regulation of extrinsic apoptotic signaling pathway in absence of ligand
Process(es)
protein kinase activityprotein serine/threonine kinase activitydeath receptor bindingprotein bindingATP bindingubiquitin protein ligase bindingprotein complex bindingidentical protein bindingdeath domain binding
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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